Analysis Note

Positive ControlA-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue

Application

Frozen Sections (1:50-1:100)Immunoblotting (1:250-1:500)Immunofluorescence (1:50-1:100)Immunoprecipitation (10 l/mg protein lysate)Neutralization Studies (see comments)Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)

General description

This Anti-DNA-PK (Ab-2) Mouse mAb (18-2) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of DNA-PK (Ab-2).

Recognizes the ~470 kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing the splenocytes with mouse myeloma SP2/0 cells (see application references). Recognizes the ~460 kDa DNA-PK protein.

Immunogen

Epitope: within amino acids 1-2713 of DNA-PK

Human

DNA-PK purified from HeLa cells

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kharbanda, S., et al. 1997. Nature386, 732.McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.Peterson, S.R., et al. 1997. J. Biol. Chem.272, 10227.Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol.8, 153.Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem.271, 8936.Connelly, M.A., et al. 1996. Gene175, 271.Danska, J.S., et al. 1996. Mol. Cell. Biol.16, 5507.Han, Z., et al. 1996. J. Biol. Chem.271, 25035.Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.Song, Q., et al. 1996. EMBO J.15, 3238.Teraoka, H., et al. 1996. FEBS Lett.393, 1.Blunt, T., et al. 1995. Cell80, 813.Hartley, K.O., et al. 1995. Cell82, 849.Kirchgessner, C.U., et al. 1995. Science267, 1178.Lees-Miller, S.P., et al. 1995. Science267, 1183.Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.

This antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Reconstitution

For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)